Journal: Marine Drugs

Article Title: In Search of the Role of Three-Finger Starfish Proteins

doi: 10.3390/md22110488

Figure Lengend Snippet: ELISA analysis of the Lystar5 interaction with immobilized membrane fraction of different tissues of A. rubens . Parameters describing the curve fit for coelomocytes: A 1 = 1.97 ± 0.09, EC 50 = 54.5 ± 15.4 nM, nH = 0.71 ± 0.1; for coelomic epithelium: A 1 = 2.85 ± 0.12, EC 50 = 13.4 ± 6.6 nM, nH = 0.75 ± 0.10. Curves were not fitted for the body wall and arm tip. Data present as the background-subtracted data, O.D. ± SEM (n=5).

Article Snippet: Then, plates were blocked by 5% BSA in PBS for 2 h (RT), washed (ELISA wash buffer, Mybiosource, San Diego, CA, USA), incubated with different concentrations of Lystar5 (10 −5 –10 −10 M) for 2 h, washed, incubated with anti-Lystar5 serum (1:100), for 1 h, washed, incubated with donkey anti-mouse HRP-conjugated antibody (Jackson Immunoresearch, 715-035-150, 1:1000) for 1 h, washed, and incubated with TMB-One ELISA Substrate (Mybiosource).

Techniques: Enzyme-linked Immunosorbent Assay, Membrane